University of Connecticut |
Education: Ph.D. Ohio State University, Postdoctoral study, University of Virginia Research Interests: Transgenic, knockout and genetic approaches are used to study mechanisms of muscle development in the mouse, with an emphasis on regulation of cell fates and mechanisms of gene expression. Our long-term goal is to define the genetic pathways that culminate in the activation of muscle regulatory factors in muscle precursor cells during development, an understanding of which will provide insight into how embryonic cells choose between alternative cell fates during development. A second major research area focuses on the biology of stem cells resident in muscle tissue. Following injury of adult skeletal muscle, or in diseases such as Duchenne muscular dystrophy, skeletal muscle undergoes a regenerative process that in many ways resembles muscle development in the embryo. Skeletal muscleÕs enormous regenerative capacity is mediated by muscle satellite cells, normally quiescent stem cells that are ÒactivatedÓ in response to injury or disease. Despite their essential function, key aspects of satellite cell biology remain unresolved, including their developmental origin, potential and regulation of commitment to myogenesis. Our work currently focuses on the use of cell marking experiments and mouse genetics to investigate these fundamental aspects of satellite cell biology. Recent evidence indicates that additional types of stem cells also exist in skeletal muscle tissue, the identification of which is essential for understanding diseases of abnormal bone formation and for developing therapies for musculoskeletal diseases. Characterization of this stem cell population and its relationship to muscle satellite cells is also being pursued. Selected Publications:
Goldhamer, D.J., Faerman, A., Shani, M., and Emerson, C.P. Jr. (1992). Regulatory elements that control the lineage specific expression of myoD. Science 256, 538-542. Litvin, J., Montgomery, M.O., Goldhamer, D.J., Emerson, C.P. Jr., and Bader, D.M. (1993). Identification of DNA binding proteins(s) in the developing heart. Dev. Biol. 156, 409-417. Woloshin, P., Song, K., Degnin, C., McNeil-Killary, A., Goldhamer, D. J., Sassoon, D., and Thayer, M. J. (1995). MSX-1 inhibits expression of MyoD in primary fibroblasts X 10T1/2 cell hybrids. Cell 82, 611-620. Faerman, A., Goldhamer, D. J., Puzis, R., Emerson, C. P., and Shani, M. (1995). The distal human MyoD enhancer sequences direct unique muscle-specific patterns of lacZ expression during mouse development. Dev. Biol. 171, 27-38. Goldhamer, D. J., Brunk, B., A. Faerman, A. King, Shani, M., and Emerson, C. P. Jr. (1995). Embryonic activation of the myoD gene is regulated by a highly conserved distal control element Development 121, 637-649. Brunk, B. P., Goldhamer, D. J., and Emerson, C. P. Jr. (1996). Regulated demethylation of the myoD distal enhancer during skeletal myogenesis. Dev. Biol. 177, 490-503. Kucharczuk, K. L., and Goldhamer, D. J. (1997). Nuclear DNA binding proteins. Methods in Cell Biology (Charles P. Emerson Jr. and H. Lee Sweeney, eds). Vol. 52: 439-472. Academic Press. Kablar, B., Asakura, A., Krastel, K., Ying, C., May, L., Goldhamer, D. J., and Rudnicki, M. A. (1998). MyoD and Myf-5 define the specification of musculature of distinct embryonic origin. Biochem. Cell Biol. 76, 1079-1091. Kablar, B., Krastel, K., Ying, C., Tapscott, S. J., Goldhamer, D. J., and Rudnicki, M. A. (1999). Myogenic determination occurs independently in somites and limb buds. Dev. Biol. 206, 219-231. Kucharczuk, K. L., Love, C. M., Dougherty, N. M., and Goldhamer, D. J. (1999). Fine-scale transgenic mapping of the MyoD core enhancer: MyoD is regulated by distinct but overlapping mechanisms in myotomal and non-myotomal muscle lineages. Development 126, 1957-1965. Chen, J. C. and Goldhamer, D. J. (1999). Transcriptional mechanisms governing MyoD expression in the mouse. Cell and Tissue Research. (Rolf Zeller, ed.). 296, 213-219. Chen, J. C., Love, C. M., and Goldhamer, D. J. (2001). Two upstream enhancers collaborate to regulate the spatial patterning and timing of MyoD transcription during mouse development. Dev. Dyn. 221, 274-288. Andreucci, J. J., Grant, D., Cox, D. M., Tomc, L. K., Prywes, R., Goldhamer, D. J., Rodrigues, N., Bedard, P.-A., and McDermott, J. C. (2002). Composition and function of AP-1 transcriptional complexes during muscle cell differentiation. J. Biol. Chem. 277: 16426-16432. Chen, J. C., Ramachandran, R., and Goldhamer, D. J. (2002). Essential and redundant functions of the MyoD distal regulatory region revealed by targeted mutagenesis Dev. Biol. 245, 213-223. Chen, J. C. and Goldhamer, D. .J. (2003). Skeletal muscle satellite cells. Reproductive Biology and Endocrinology 1:101 Chen, J. C. and Goldhamer, D. J. (2004). The core enhancer is essential for the proper timing of MyoD activation in limb buds and branchial arches. Dev. Biol. 265, 502-512 Chen, J. C. J., Mortimer, J., Marley, J. and Goldhamer, D. J. (2005). MyoD-cre transgenic mice: a model for conditional mutagenesis and lineage tracing of skeletal muscle. Genesis 41, 116-121. Yamamoto, M., Watt, C., Schmidt, R., Kuscuoglu, U., Miesfeld R., and Goldhamer, D. J. (2007). Cloning and characterization of a novel MyoD enhancer binding factor. Mech. Dev. 124, 715-728. Kirillova,I., Gussoni, E., Goldhamer, D. J., and Yablonka-Reuveni, Z. (2007). Myogenic reprogramming of retina-derived cells following their spontaneous fusion with myotubes. Dev. Biol. 311, 449-463. O¹Rourke, J. R., Georges, S. A., Seay, H. R., McManus, M. T., Goldhamer, D. J., Tapscott, S. J., Swanson, M. S., and Harfe, B. D. (2007). Dicer is required for embryonic myogenesis. Dev. Biol. 311, 359-368. Yamamoto, M., Shook, N. A., Kanisicak, O., Yamamoto, S., Wosczyna, M. N., Camp, J. R., and Goldhamer, D. J. (2009). A multifunctional reporter mouse line for Cre- and FLP-dependent lineage analysis. Genesis 47, 107-114. Lounev, V. Y., Ramachandran, R., Wosczyna, M. N., Yamamoto, M., Maidment, A. D. A., Shore, E. M., Glaser, D. L., Goldhamer, D. J., and Kaplan, F. S. (2009). Identification of progenitor cells that contribute to heterotopic skeletogenesis. J Bone Joint Surg 91: 652-663 Kanisicak, O., Mendez, J. J., Yamamoto, S., Yamamoto, M., and Goldhamer, D. J. (2009). Progenitors of skeletal muscle satellite cells express the muscle determination gene, MyoD. Dev. Biol. 332, 131-141. |
