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Associate Professor James L. Cole
Education: Ph.D., University of California; Post-doctoral study, Stanford University.
Research Interests: Characterization of macromolecular interactions using analytical ultracentrifugation and related biophysical techniques. Double-stranded RNA activated protein kinase (PKR); interferon antiviral defense pathway; trp RNA-binding attenuation Protein (TRAP).
Selected Publications:
Anderson, E., Quartararo, C., Brown, R.S., Shi, Y., Yao, X. and Cole, J.L (2010) Analysis of monomeric and dimeric phosphorylated forms of protein kinase R. Biochemistry 10.1021/bi901873p
Zhang, N., Wang, Q. Ehlinger, A., Randles, L., Lary, J. W., Kang, Y., Haririnia, A., Storaska, A. J., Cole, J. L., Fushman, D., Walters, K. J. (2009) Structure of the S5a:K48-Linked Diubiquitin Complex and Its Interactions with Rpn13. Mol. Cell 35, 280-290.
VanOudenhove, J., Anderson, E., Krueger, S. and Cole, J.L. (2009) Analysis of PKR structure by small angle scattering.
Snyder, S., Lary, J., Chen, Y., Gollnick, P. and Cole, J.L. (2004) Interaction of the Trp RNA-binding Attenuation Protein (TRAP) with Anti-TRAP. J. Mol. Biol. 338, 669-682.
Lemaire, P.A., Lary, J. and Cole, J.L. (2005) Mechanism of PKR activation: Dimerization and kinase activation in the absence of double-stranded RNA. J. Mol. Biol. 345, 81-90.
Lemaire, P.A., Tessmer, I., Craig, R., Erie, D.A. and Cole, J.L. (2006) Unactivated PKR exists in an open conformation capable of binding nucleotides. Biochemistry 45, 9074-9084.
Ucci, J. W., Kobayashi, Y., Choi, G., Alexandrescu, A. and Cole, J. L. (2007) The mechanism of interaction of the dsRNA binding domain of PKR with short dsRNA sequences. Biochemistry 46, 55-65.
Yan, Q., Malashkevich, V.N., Fedorov, A., Fedorov, E., Cao, E., Lary, J.W., Cole, J.L., Nathenson, S.G. and Almo S.C. (2007) Structure of CD84 provides insight into SLAM family function. Proc. Natl. Acad. Sci. USA 104, 10583-10588.
Cole, J.L. (2007) Activation of PKR: An open and shut case? Trends Biochem. Sci. 32, 57-62.
Cole, J.L., Lary, J.W., Moody, T. and Laue, T.M. (2008) Analytical Ultracentrifugation: Sedimentation Velocity and Sedimentation Equilibrium. In Methods in Cell Biology (Correia, J.J. and Dietrich, H.W., Eds.). Elsevier.
Robertson, P.D., Warren, E.M., Zhang, H., Friedman, D.B., Lary, J.W., Cole, J.L., Tutter, A.V., Walter, J.C., Fanning, E., Eichman, B.F. (2008) Domain architecture and biochemical characterization of vertebrate MCM10. J. Biol. Chem. 83, 3338-3348.
Tyukhtenko, S., Deshmukh, L., Kumar, V., Lary, J. Cole, J.L., Lemmon, V. and Vinogradova, O. (2008) Characterization of neuron specific L1-CAM cytoplasmic tail: naturally disordered in solution it exercises different binding modes for different adaptor proteins. Biochemistry 47, 4160-4168.
Anderson, E. and Cole, J.L. (2008) Domain stabilities in PKR: Evidence for weak interdomain interactions. Biochemistry 47, 4887-4897
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University of Connecticut |